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The shop is co-owned by tattoo artists Ami James and Chris Nuñez, and also features artists Chris Garver, Darren Brass and Yoji Harada. California artist Kat Von D joined the shop during the first season while Brass had an injured arm. At the end of season two, however, Kat left the show after a series of escalating personal differences with Ami James. She is slated to appear in her own Miami Ink spinoff entitled LA Ink. Each episode features a number of customers along with their backstories and motivations for choosing their tattoos. There is also some focus on the personal lives of the artists.

The following people have appeared on Miami Ink:

• Evan Seinfeld - Musician - Biohazard
• Sunny Garcia - Pro surfer
• Mark Zupan - Athlete - Murderball
• H2O - Band
• Phil Varone - Musician - Skid Row/Saigon Kick
• Anthony Bourdain - Chef/Author/Television personality
• Harold Hunter - Skateboarder
• Lloyd Banks - Rapper
• Johnny Messner - Actor
• Ivy Supersonic - Fashion designer
• Chris Jacobs - Host of TLC Overhaulin'
• Reginald Arvizu Musician - Korn
• Craig Ferguson - Television Host/Actor
• A. B. Quintanilla - Mexican American musician and producer
• Paul Teutul, Sr. - American Chopper
• Paula Meronek - MTV The Real World
• Chase Lisbon - Photographer/creator of adult website Supercult.Com
• Diablo Dimes - Musician
• Mickdeth - Musician / Bassist of Eighteen Visions

• Official TLC Site: Miami Ink
• Tattoo Finder: TLC Miami Ink
• Miami Ink Links

• Wikipedia: Miami Ink
• TV.com: Miami Ink