After Poco hears that Peggy has gone out to search for him, Dio sends a zombie named Doobie to attack Peggy after she refuses to join his legion. Luckily, Jonathan and the others arrive in time to save her. Doobie soon reveals that his head is filled with poisonous snakes, managing to deliver a few bites to Jonathan's face, but neither the venom nor Doobie stands up to Jonathan's power. With Poco and Peggy reunited, Jonathan goes on to face Dio. Dire decides to face him first and launches an attack, but he is caught by Dio's freezing ability and is shattered into pieces, though with his last breath, he manages to shoot a ripple-powered rose into Dio's eye. As Jonathan learns from that sacrifice how he can damage Dio using the sword he received from Bruford, he goes on to confront Dio whilst Tonpetty and Straizo take on his undead army. Jonathan manages to slice his sword straight through Dio's body, but Dio uses this opportunity to freeze his sword and attack Jonathan's neck. However, Jonathan manages to thaw himself by heating the sword with a torch, though Dio manages to protect himself from Jonathan's attack. Just then, Jonathan sets his fists on fire to counter Dio's freezing and manages to break through his defenses with an Overdrive attack. As his body melts and he falls into the abyss, Dio launches a final attack which pierces Jonathan's hand, but appears not to do much more. With Dio presumed dead, Jonathan takes a long earned rest from his battles.