After the fight between Ryuko and Tsumugu is dissolved thanks to Mako and Senketsu, Aikuro explains to her how Tsumugu's sister, Kinue, died in a Life Fiber experiment, going on to explain that Senketsu was made with Ryuko's DNA, hence why only she can wear and communicate with him. Meanwhile, Honnouji Academy makes preparations for a Cultural and Sports Grand Festival to celebrate Ragyo visiting the school. Learning that this a cover up for a final experiment in which all of Honnouji City will be sacrificed to the Life Fibers, Ryuko and the others head for Honnouji, where the ceremony has begun. As Ragyo begins having the Life Fibers devouring everyone in the stadium, Ryuko and company arrive on the scene. Just then, Satsuki stabs Ragyo and announces her rebellion against her and the Life Fibers, revealing this to be the purpose she has built Honnouji Academy for.