Lupin investigates the news of a fake version of one of his trademark calling cards during a high-class party. A group of assassins, the Tarantula, crash the party and murder the host. Zenigata, investigating the card, chases Lupin but ends up shot and injured by a silver Walther P38. Lupin, catching a glimpse of the familiar gun, is determined to avenge not only Zenigata, but an episode of his past. He and Fujiko infiltrate the island by joining Tarantula, while Jigen and Goemon hide in the underground. Lupin also befriends Elen, an assassin who is disillusioned with Tarantula following the death of her younger brother who was also with the organization. Prevented from leaving by an ingenious poison and also interested in Tarantula's large stock of gold, Lupin and his gang work to bring down the assassins and find the bearer of the silver Walther.